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Time- and dose-dependent production of MCP-1 by HUVEC in response to B. burgdorferi or IL-1. Conditioned media were collected from HUVEC incubated with medium alone (Unstim), sham preparations, 5 U of IL-1 per ml, or B. burgdorferi at a ratio of 1, 10 or 100 Bb/EC for the indicated times. Amounts of MCP-1 were measured by ELISA. Datum points represent the means ± SD of three replicate samples. This experiment was repeated once with similar results.
