TABLE 2.
Effect of IL-10 on production of MCP-1 by HUVEC
| Stimulusa | MCP-1 produced (ng/106 EC)
|
||
|---|---|---|---|
| No IL-10 | +IL-10 (2 ng/ml) | +IL-10 (20 ng/ml) | |
| None | 9.5 ± 1.2 | NDb | 12.6 ± 0.7c |
| IL-1 (0.1 U/ml) | 52.9 ± 6.3 | ND | 59.3 ± 1.8 |
| IL-1 (5 U/ml) | 100.9 ± 6.2 | ND | 101.8 ± 1.4 |
| B. burgdorferi | 32.7 ± 1.2 | 23.8 ± 3.1d | 22.7 ± 0.7e |
a
HUVEC were incubated with IL-1 or B. burgdorferi at a ratio of 10 Bb/EC for 8 h with or without addition of IL-10. MCP-1 was measured by ELISA.
b
ND, not determined.
c
Significantly greater than without IL-10; P = 0.02.
d
38% inhibition of B. burgdorferi-stimulated increase; P < 0.01.
e
43% inhibition of B. burgdorferi-stimulated increase; P < 0.0003.