Figure 4.

Pattern-violating Gabor sequences result in different ΔF/F and USI changes in different imaging planes. A, Example session 1 ΔF/F response traces for individual compartment (left to right: L2/3-D, L2/3-S, L5-D and L5-S) ROIs with high (top), null (middle) or low (bottom) USIs. Mean ± standard error of the mean (SEM) ΔF/F across Gabor sequences is plotted. Dashed vertical lines mark onset of D/U images. B, Example USI null distribution for one ROI from L2/3-S in session 1, generated by shuffling D-G and U-G labels for the same ROI and recomputing the shuffled USIs 10⁴ times. Significant regions highlighted in red, and true USI value labeled in blue. C, USI percentile distributions for each plane for all session 1 ROIs. Dashed horizontal lines depict null hypotheses (i.e., uniform distribution). Significant regions highlighted in red (p < 0.05, shuffle test as shown in B). D, Percentage ± bootstrapped standard deviation (SD) of significant USIs for all segmented ROIs in session 1 for each plane. All sequences (any mean orientation) are included in the analysis. E, Same as D, but restricted to Gabor sequences with mean orientations shared between D and U images . F, Gabor sequence stimulus USIs for all tracked ROIs. Each line represents a single ROI’s USIs over all three sessions. G, Mean (± SEM) across the absolute values of the Gabor sequence stimulus USIs for tracked ROIs, as shown in F. H, Variance (± bootstrapped SD) across the Gabor sequence stimulus USIs for tracked ROIs, as shown in F. *p < 0.05, **p < 0.01, ***p < 0.001 (two-tailed, corrected). See Table 1 for details of statistical tests and precise p-values for all comparisons.