(A-C) Refinement of a small subset of the GPCR dataset. (D-F) Refinement of a small subset of the SARS-COV2 dataset. (A, D) “Gold-standard” FSC curves of the reconstruction using voxel-based refinement (gray), global GMM-based refinement (yellow) and patch-by-patch refinement (pink). (B, E) Overall structure of the initial reconstruction and the final patch-by-patch refinement result, colored by local resolution. (C, F) Comparison of local features from the voxel-based, global GMM-based and patch-by-patch refinement.