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. Author manuscript; available in PMC: 2024 Feb 12.
Published in final edited form as: J Autoimmun. 2022 Nov 17;137:102946. doi: 10.1016/j.jaut.2022.102946

Figure 6. Retention of functional properties by P364L RelB.

Figure 6.

(A) RelA and P364L RelB both undergo nuclear transit in response to LIGHT in patient fibroblasts. Nuclear lysates of LIGHT-stimulated patient (P364L) and control (C) cells (100ng/ml) were immunoblotted for RelB. Null = complete RelB deficient patient fibroblasts. (B) P364L RelB in nuclear lysates of patient EBV-B cell lines binds a double-stranded consensus NFκB DNA binding sequence, but not a mutated control sequence. CONS = double stranded NFκB consensus sequence oligonucleotide. M = mutated non-functional double stranded NFκB consensus sequence oligonucleotide. Blotting of input nuclear lysate for RCC1 was used as a loading control.