TABLE 2.
Expression of apyrase activity by EIEC and S. flexneri strains
| Strain | Relevant genotype | Apyrase activitya at:
|
Ratio of apyrase activity (37°C/30°C) | |
|---|---|---|---|---|
| 30°C | 37°C | |||
| HN280 | Wild-type EIEC | 0.146 | 0.925 | 6.3 |
| HN680 | Δhns | 0.665 | 0.946 | 1.4 |
| HN280-3 | ΔvirF | 0.034 | 0.042 | 1.2 |
| HN280-3(pMYSH6504) | ΔvirF pvirF | 0.123 | 0.754 | 6.1 |
| HN280-8 | ΔvirF | 0.072 | 0.145 | 2.0 |
| HN280-8(pMYSH6504) | ΔvirF pvirF | 0.187 | 0.691 | 3.7 |
| HN280-16 | ΔvirB | 0.022 | 0.028 | 1.3 |
| HN280-16(pBN1) | ΔvirB pvirB | 0.113 | 0.654 | 5.8 |
| HN280-35 | ΔvirB | 0.016 | 0.036 | 2.2 |
| HN280-35(pBN1) | ΔvirB pvirB | 0.086 | 0.806 | 9.4 |
| HN280/32 | apy (chromosome) | 0.090 | 0.238 | 2.6 |
| HN680/32 | Δhns | 0.718 | 0.796 | 1.1 |
| SFZM53 | Wild-type S. flexneri serotype 4 | 0.144 | 0.736 | 5.1 |
| SFZM53-1 | Δ(virF phoN-Sf) | 0.026 | 0.112 | 4.3 |
| SFZM53-1(pMYSH6504) | Δ(virF phoN-Sf) pvirF | 0.095 | 1.168 | 12.3 |
| SFZM53-15 | ΔvirB | 0.034 | 0.052 | 1.5 |
| SFZM53-15(pBN1) | ΔvirB pvirB | 0.103 | 0.851 | 8.3 |
| M90T | Wild-type S. flexneri serotype 5 | 0.134 | 0.665 | 5.0 |
| BS176 | pINV cured | ND | ND | |
| 454 | Wild-type S. flexneri serotype 2a | 0.053 | 0.692 | 13.0 |
a
Expressed as AU obtained by scanning of developed zymograms as described in Materials and Methods. Results are averages for at least four independent experiments. Standard errors were less than 15%. ND, not detectable.