Fig. 2. Osteoblasts were increased and bone formation was enhanced in Kdm7a cKO mice.

ALP IHC staining was performed on the sections from 24-week-old female (A, B) and male (C, D) mice (arrows indicate osteoblasts). Image scale in (A, C): 20 μm. The number of osteoblasts was counted. Serum PINP levels were measured in female and male mice using ELISA (E, F). Calcein double labelling was performed. MAR, MS/BS and BFR/BS were measured and calculated for non-decalcified sections from 24-week-old female mice (G–J). Image scale in (G): 20 μm. Data are presented as box-and-whiskers plots, n = 8 in (B, D), n = 10 in (E, F), n = 7 in (H–J). Student’s t test was performed for statistical analyses. *p < 0.05, **p < 0.01, ***p < 0.001.