Fig. 7. KDM7A regulated Fap and Rankl expression by demethylating H3K9me2 and H3K27me2 on Fap and Rankl promoters.

The cell lysates from long bone BMSCs containing chromatin complexes were incubated with anti-KDM7A, anti-H3K9me2, anti-H3K27me2 antibodies or IgG, then the immunocomplexes were captured. ChIP-qPCR was performed to detect the presence of KDM7A, H3K9me2 and H3K27me2 on the promoter of Fap (A–C) or Rankl (D-F). The amplification of a fragment 5 kb downstream of the transcription start site was used as a control. The protein levels of the major components of Wnt/β-catenin pathway were measured in undifferentiated BMSCs using Western blotting (G). Data are presented as box-and-whiskers plots, n = 5. Comparisons were conducted using Student’s t test. **p < 0.01, ***p < 0.001, ns: no significance.