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. 2023 Sep 6;61(2):935–949. doi: 10.1007/s12035-023-03613-3

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© The Author(s) 2023, corrected publication 2024

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Fig. 4 — Overexpression of GAS5 prevented the effect of miR-21 mimic on SCs proliferation, migration, and apoptosis. A CCK-8 assay was used to examine the proliferation of SCs. B Crystal violet staining was used to trace the migration ability of SCs. C The number of cell migration statistics analysis. D TUNEL assay was used to detect the number of apoptotic SCs (green). Scale bar = 50 μm. E The statistics analysis apoptosis index of SCs. F Western blot was used to detect the expression of cleaved caspase-3/Bcl-2/BAX and GAPDH. G Statistics analysis of gray value for protein bands. For the above, data are represented as mean ± SD (two-way ANOVA, Tukey’s post hoc test: *P < 0.05, **P < 0.01, ***P < 0.001)