Fig. 6.

GAS5 negatively regulates miR-21 expression, and miR-21 is the direct target of GAS5. A RT-qPCR was used to detect the expression of GAS5 with different siRNA segments in SCs. B RT-qPCR was used to detect the level of miR-21 with different knocking efficiency of GAS5 in SCs. C RT-qPCR was used to detect the expression of GAS5 when infected lentivirus expression of GAS5 in SCs. D RT-qPCR was used to detect the expression of miR-21 when infected lentivirus that expression of GAS5 in SCs. E Subcellular fractionation assay measured the localization of GAS5 in SCs. F The binding sites between GAS5 and miR-21 are depicted in this diagram. G The dual-luciferase reporter gene assay was used to detect the luciferase activity of GAS5 when SCs transfected miR-21 mimic or NC. For the above, data are represented as mean ± SD (one-way ANOVA, Scheffe’s post hoc test: *P < 0.05, **P < 0.01, ***P < 0.001)