TABLE 2.
Cytokine production by lung T-cell clones
| Clone | Concn (pg/ml)a
|
|||||||
|---|---|---|---|---|---|---|---|---|
| IFN-γ
|
IL-4
|
IL-5
|
IL-10
|
|||||
| Ag | Control | Ag | Control | Ag | Control | Ag | Control | |
| I-G11L | 10,300 | 0 | 272 | 0 | 195 | 0 | 1,850 | 0 |
| I-F4L | 20,200 | 5,000 | 0 | 0 | 35 | 0 | 0 | 0 |
| I-G8L | 4,900 | 1,100 | 0 | 0 | 0 | 0 | 1,850 | 1,570 |
| I-A3L | 0 | ND | 290 | ND | 0 | ND | 587 | ND |
| I-D6L | 0 | 0 | 0 | 0 | 0 | 0 | 792 | 0 |
a
T cells (6 × 105) were cocultured for 48 h with 106 irradiated splenic APCs with (Ag) or without (Control) antigen (10 μg of H37Rv sonicate per ml). Cytokine contents in two independently obtained sets of supernatants of each clone were measured by ELISA in triplicate (standard deviation of <10% among triplicate determinations). Positive results obtained in two independent experiments differed less than 25%. 0, concentration lower than the sensitivity of the ELISA. ND, not determined.