Figure S3.

N24A and R30A mutations of CI accessory subunit NDUFB4 modify cellular metabolism and substrate preference. A, the rate of ATP formation from OXPHOS and glycolysis and OXPHOS, n = 3 (B) The glycolytic index, n = 3 from independent experiments. C, confocal microscopy of cells stained with TOMM20 and nuclei (Hoechst). D–F, enzymatic activity of (D) Citrate synthase, (E) Lactate dehydrogenase (LDH), and (F) Malate dehydrogenase, n = 6-7 biological replicates. G, contributions of N-linked respiration and S-linked respiration in the co-stimulated protocol, n = 5-6 from independent experiments. H and I, catalytic activity of (H) Complex I (CI) and (I) Complex II, n = 6-7 biological replicates. (J) Steady-state CoQ10 levels, n = 7-9 biological replicates. Values are Means ± SEM. Comparisons between groups were determined using a one-way ANOVA with Tukey post-hoc test. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.