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Workflow for cell stimulation and mitochondrial protein biotinylation
Cells stably expressing mitochondria-localized TurboID are stimulated with lipopolysaccharide (LPS) and interferon gamma (IFNγ) to activate an inflammatory response. As a result of stimulation, specific mitochondrial cysteines undergo oxidation. All cells are then treated with biotin to initiate biotinylation of mitochondrial proteins via the biotin ligase TurboID.
