Table 1.

Recipe for click master mix

Reagent	Stock concentration	Solvent used	Amount of stock (for 1 click reaction)	Final concentration in click reaction (after addition to 200 μL peptide sample)
TBTA	1.7 mM	See “materials and equipment”	12 μL	0.102 mM
Copper sulfate	50 mM	Molecular biology grade H2O	4 μL	1 mM
Biotin-azide	200 mM	DMSO	4 μL	4 mM
Sodium ascorbate	250 mM	Molecular biology grade H2O	4 μL	5 mM