Table 5.
Liquid chromatography gradient for separation of peptides
| Time | Duration | Flow (nL/min) | Mobile phase B (percentage of solvent) |
|---|---|---|---|
| 00:00 | 00:00 | 300 | 3 |
| 05:00 | 05:00 | 220 | 10 |
| 15:00 | 10:00 | 220 | 20 |
| 64:00 | 49:00 | 220 | 47 |
| 66:00 | 02:00 | 250 | 95 |
| 70:00 | 04:00 | 250 | 95 |
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