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Click master mix
| Reagent | Stock concentration | Solvent used | Amount of stock (for 1 click reaction) | Final concentration in click reaction |
|---|---|---|---|---|
| TBTA | 1.7 mM | See TBTA recipe below | 12 μL | 0.102 mM |
| Copper sulfate | 50 mM | Molecular biology grade H2O | 4 μL | 1 mM |
| Biotin-azide | 200 mM | DMSO | 4 μL | 4 mM |
| Sodium ascorbate | 250 mM | Molecular biology grade H2O | 4 μL | 5 mM |
