Figure 4.

FXN protein distribution in the heart

(A) Heart tissue samples showing FXN expression (IHC) of vehicle-treated WT and FXN-MCK mice and AAV-treated FXN-MCK mice. The images in the bottom row depict the magnification of an area from the sample directly above it (blue box). Scale bars represent 1 mm (top row) and 100 μm (bottom row). (B) HALO analysis to semiquantify the mean percentage of areas exhibiting FXN stains in strong, moderate, and weak intensity, and total (adding all percentages of strong, moderate, and weak) of all mice. The data below the graph show the mean total percentages of areas exhibiting FXN stains at any intensity in each group, corresponding to the bars above; SDs are provided in Table S7.