Figure 3. Validation of GPX3-dependent GDF15 gene expression.

A. Stable shRNA mediated knock-down of GPX3 in OVCAR3 leads to decreased GDF15 mRNA expression (n=3, One Way ANOVA, Tukey’s post-test).

B. shRNA-mediated knock-down of GPX3 in OVCAR4 cells results in decreased GDF15 mRNA expression (n=3, One Way ANOVA, Tukey’s post-test).

C. Transient siRNA-mediated GPX3 knock-down in OVCA433 cells leads to decreased GDF15 mRNA expression (n=3, t-test).

D. GDF15 mRNA expression in tumors from ID8 cells (from Figure 1), assessed by semi-quantitative real time RT-PCR (Kruskal-Wallis test).

E. shRNA mediated knock-down of GPX3 in OVCAR3 cells decreases intracellular and

F. Extracellular GDF15 protein expression (n=3, One Way ANOVA, Tukey’s post-test).