Table 2.
Primer sequences used for semi-quantitative real-time RT-PCR.
| Primer | Sequence |
|---|---|
| Human 18S Forward | 5’-AGAAACGGCTACCACATCCA-3’ |
| Human 18S Reverse | 5’-CACCAGACTTGCCCTCCA-3’ |
| Human GAPDH Forward | 5’-GAGTCAACGGATTTGGTCGT-3’ |
| Human GAPDH Reverse | 5’-TTGATTTTGGAGGGATCTCG-3’ |
| Human GPX3 Forward | 5-GGGGAT GT CAAT GGAGAGAA-3’ |
| Human GPX3 Reverse | 5’-TTCATGGGTTCCCAGAAGAG-3’ |
| Mouse GAPDH Forward #1 (Fig1A) | 5’-ATGTGTCCGTCGTGGATCTGAC-3’ |
| Mouse GAPDH Reverse #1 (Fig1A) | 5’-AGACAACCTGGTCCTCAGTGTAG-3’ |
| Mouse GAPDH Forward #2 (Fig3–5) | 5’-AGGTCGGTGTGAACGGATTTG-3’ |
| Mouse GAPDH Reverse #2 (Fig3–5) | 5’-GGGGT CGTT GAT GGCAACA-3’ |
| Mouse GPX3 #2 Forward (Fig1A) | 5’-AGGAGATGTGAACGGGGAGA-3’ |
| Mouse GPX3 #2 Reverse (Fig1A) | 5’-GGGAGGGCAGGAGTTCTTCA-3’ |
| Mouse GPX3 #3 Forward (Fig3–5) | 5’-GACAT CCGCT GGAACTTT GA-3’ |
| Mouse GPX3 #3 Reverse (Fig3–5) | 5’-CCATCTTGACGTTGCTGACT-3’ |
| Human GDF15 Forward | 5’-GCAAGAACTCAGGACGGTGA-3’ |
| Human GDF15 Reverse | 5’-TGGAGTCTTCGGAGTGCAAC-3’ |
| Human EREG Forward | 5’-GT GATT CCAT CAT GTAT CCCAGG-3’ |
| Human EREG Reverse | 5’-GCCATT CAT GT CAGAGCT ACACT-3’ |
| Human ITGB3 Forward | 5’-ATTCCACACCCTCACTGCTG-3’ |
| Human ITGB3 Reverse | 5’-GAAGCATAGGGCCAGACCTC-3’ |
| Human IL6 Forward | 5’-CCTGAACCTTCCAAAGATGGC-3’ |
| Human IL6 Reverse | 5’-TTCACCAGGCAAGTCTCCTCA-3’ |
| Human PCDH7 Forward | 5’-GACTCTGGGCGTCTCTGAAG-3’ |
| Human PCDH7 Reverse | 5’-CTCAACTCCGACTCTGCTCA-3’ |
| Human IL8 Forward | 5’-ACTGAGAGTGATTGAGAGTGGAC-3’ |
| Human IL8 Reverse | 5’-AACCCTCTGCACCCAGTTTTC-3’ |
| Mouse FOXP3 Forward | 5’-GGCCCTT CT CCAGGACAGA-3’ |
| Mouse FOXP3 Reverse | 5’-GCT GAT CAT GGCT GGGTT GT-3’ |
| Mouse PD-1 Forward | 5’-TGGGGCCTAAGCCTATGTCT-3’ |
| Mouse PD-1 Reverse | 5’-CTCCCAAGGGTGGCTTTAGG-3’ |
| Mouse PD-L1 Forward | 5’-ACTTGCTACGGGCGTTTACT-3’ |
| Mouse PD-L1 Reverse | 5’-CTCTCCCCCTGAAGTTGCTG-3’ |