Figure 3. Reliable propagation of action potentials at axonal branching point.

A: Time-averaged image of GCaMP6s activity during electrical stimulation. Scale bar: 5 μm. B: Color map of parent (0) and two secondary branches (1 and 2). C: Maps of normalized peak amplitudes at each frequency. D: Normalized GCaMP6s/mRuby3 signal for each branch at different frequency. Average of 5 trials. Colors according to branches in 1B. E: Peak amplitudes of signals in 3D as a function of frequency. F: Area under curve of signals in 3D as a function of frequency. Vertical lines show standard error. G: Percentage of signals that propagate at each branch, as a function of frequency.