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[Preprint]. 2024 Feb 15:2024.01.31.578281. Originally published 2024 Feb 4. [Version 2] doi: 10.1101/2024.01.31.578281

Figure 2: CII-synNotch MSCs detect degradation of cartilage tissue.

Figure 2:

(A) 3 mm-diameter primary porcine cartilage explants were enzymatically treated or cryosectioned prior to enzymatic treatment. Enzyme treatment reveals CII epitopes detected by the mAbCII recognition motif. (B) SynNotch-inducible SEAP expression after plating engineered cells on enzymatically-damaged whole-plug explants (t=72 hr, Student’s t-test on n=3 replicates per group). (C) Inducible luciferase expression from engineered cells on cryosections treated with varying concentrations of trypsin-EDTA (t=48hr). (D) Representative images of synNotch activation on sagittal cryosection samples without treatment and with 0.25% trypsin-EDTA (t=48hr). iRFP is false-colored as blue to enable reporter discrimination in the merged image. Scale = 200 μm. Data plotted as mean ± SEM. *p<0.05, **p<0.01.