Figure 4. Antibodies from semi-synthetic conjugates bind to C. neoformans cells.

Mu1 and H99 were cultured for 48 h in capsule-inducing media and immunofluorescence was performed using the mouse serum from mice treated with 10μg DECA-CRM197 or 10μg DECA-PA63 conjugate. Cells were imaged for Uvitext2b (Blue channel), secondary antibodies (IgG and IgM) (Green channel). Left margin indicates conjugate and inside the parentheses is the strain of C. neoformans. Merged image show annular and punctate staining in immunized mouse serum dependent on strain. Scale bar is 10 μM.