Figure 5. The DECA-CRM197 conjugate vaccine elicited opsonic antibodies.

The dot represents an individual experiment, the line is the mean and error bars are standard deviation (SD). BMDM-derived macrophages were co-cultured with C. neoformans strain 24067 in the presence of 20% guinea pig serum (as a control) and 1:50 dilution of serum from 10 μg immunized mice. Cells were counted after 2 hours by using microscopy with a 40x objective. The phagocytic index (MOI 3:1) was determined by the number of internalized cryptococcal cells per 100 macrophages.