Abstract
Kinin requiring tobacco and soybean tissues incubated on a medium containing N,6-benzyladenine-8-C14 incorporated C14 into several RNA components including adenylic and guanylic acids. About 15% of the label taken up by the tissues appeared in RNA while the remainder was distributed among several metabolites in the soluble, nonpolynucleotide fraction. Tissue grown on a kinin labeled in the side chain (N,6-benzyladenine-benzyl-C14) also incorporated a small, but nevertheless repeatable, amount of radioactivity into minor RNA components.
Ultracentrifugation studies and methylated albumin chromatography indicated that the bulk of the label from benzyladenine-benzyl-C14 is in soluble RNA. Approximately 50% of the C14 in soluble RNA is in a component which has chromatographic properties like that of benzyladenine.
It is suggested that the biological action of the kinins may hinge on their providing substituted bases in RNA in tissues which through differentiation no longer synthesize RNA-methylating enzymes. As an alternative it was hypothesized that a small amount of benzyladenine was incorporated into a m-RNA, acting there as a derepressing agent, perhaps by preventing its normal repressing function.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHERRY J. H. ASSOCIATION OF RAPIDLY METABOLIZED DNA AND RNA. Science. 1964 Nov 20;146(3647):1066–1069. doi: 10.1126/science.146.3647.1066. [DOI] [PubMed] [Google Scholar]
- COMB D. G., KATZ S. STUDIES ON THE BIOSYNTHESIS AND METHYLATION OF TRANSFER RNA. J Mol Biol. 1964 Jun;8:790–800. doi: 10.1016/s0022-2836(64)80160-1. [DOI] [PubMed] [Google Scholar]
- FLEISSNER E., BOREK E. A new enzyme of RNA synthesis: RNA methylase. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1199–1203. doi: 10.1073/pnas.48.7.1199. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANDEL H. G., CARLO P. E., SMITH P. K. The incorporation of 8-azaguanine into nucleic acids of tumor-bearing mice. J Biol Chem. 1954 Jan;206(1):181–189. [PubMed] [Google Scholar]
- MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
- STARR J. L. The incorporation of amino acids into "methyl-poor" amino acid transfer ribonucleic acid. Biochem Biophys Res Commun. 1963 Jan 31;10:181–185. doi: 10.1016/0006-291x(63)90047-0. [DOI] [PubMed] [Google Scholar]