Extended Data Figure 4: DENND5A expression varies depending on the variant.

DENND5A protein expression in a, NPCs and b, lymphoblasts. Relative DENND5A mRNA expression measured by RT-qPCR in c, NPCs and d, lymphoblasts. Measurements were made with 4 technical replicates on n = 3 independent samples. Data are mean ± SEM analyzed via Kruskal-Wallis tests with Bonferroni-corrected pairwise comparisons. c, Overexpression of FLAG-DENND5A mutagenized to contain several variants influences protein stability and expression levels in HEK293T.