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. 2024 Feb 12;22:119. doi: 10.1186/s12964-024-01512-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Decreased CSCs in KMT2B and KMT2D KD reduced CSCs but increased BC late-progenitor. A MDA-MB-231 BCCs with the Oct4a-dsRED reporter vector and transduced with the KMT2D-GFP shRNA were subjected to flow cytometry to measure the relative dsRED intensity. B Percent distribution of BCC subsets after transduction with scramble-shRNA or KMT2D shRNA. C Total number of CSCs (Oct4a^high) in scramble BCCs and KMT2D knockdown BCCs. Each experiment was repeated thrice, where a *p < .05 was considered significant. D Reat time PCR for stem cell-associated genes in KMT2B and KMT2D KD BCCs. Control PCR used cDNA from BCCs with scramble shRNA. The value for scramble shRNA is assigned 1 and the experimental values are presented as the fold change. The results show three biological replicates, mean ± SD, *p < .05 vs. scramble shRNA