Fig. 6.

POSTN mediates the increase of GSC tumorigenic properties induced by HMVEC-derived EVs. A. qRT-PCR analysis of POSTN expression in GSC#61 transduced with GFP or POSTN-GFP vector (left panel). Samples were run in duplicate. Data were normalized to the GAPDH expression in the corresponding samples. WES full-length gel image of POSTN expression in GSC#61 transduced with GFP or POSTN-GFP vector (right panel). The protein expression was quantified using AUC (area under the curve) measurements generated using Compass Software. Signal intensity was normalized to the β-actin expression in the same sample. B–D. Functional in vitro assays on GSC#61 transduced with GFP or POSTN-GFP vector. Growth curve B: points and range lines at each day represent mean ± SD of at least two independent experiments in triplicate. Migration assay C: values are reported as percentage relative to GSC#61 transduced with GFP vector and shown as mean ± SD from two independent experiments in triplicate. Colony formation assay D: percent colony number values from two independent experiments in triplicate were calculated over GSC#61 transduced with GFP vector and are shown as mean ± SD. *p < 0.05 vs GFP vector (Student’s t test). E. Transdifferentiation assay: cytofluorimetric evaluation of CD34 expression. Values are reported as fold change relative to GdEC#61 transduced with GFP vector (ns, not significant)