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. 2024 Jan 24;27(2):109001. doi: 10.1016/j.isci.2024.109001
REAGENT or RESOURCE SOURCE IDENTIFIER
Biological samples

Cervico-vaginal fluid Homo sapiens N/A
Plasma samples Homo sapiens N/A

Chemicals, peptides, and recombinant proteins

LC/MS grade water Fisher Chemical Cat#51140
DL-Dithiothreitol (DTT) Sigma-Aldrich Cat#43815
2-Iodoacetamide (IAA) Sigma-Aldrich Cat#I6125
Sequencing Grade Modified Trypsin Promega Cat#V5111
Acetonitrile, LC-MS grade, 99.8% Fisher Chemical Cat#047138.M1
Formic acid Sigma-Aldrich Cat#F0507
Ammonium bicarbonate, 99% Fisher Chemical Cat#393210010

Critical commercial assays

Proximity Extention Assay, custom version. Olink Proteomics AB www.olink.com

Deposited data

Deposited data This paper https://doi.org/10.17044/scilifelab.24241237
Data published in another paper Gutiérrez, A. L. et al.20 Identification of Candidate Protein Biomarkers for CIN2+ Lesions from Self-Sampled, Dried Cervico–Vaginal Fluid Using LC-MS/MS. Cancers 2021, Vol. 13, Page 259213, 2592 (2021). https://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD026064

Software and algorithms

MaxQuant software, version 1.5.1.2 https://www.biochem.mpg.de/6304115/maxquant https://www.maxquant.org/
R version 4.2.2 R Core Team (2022) https://www.R-project.org/
Adobe Illustrator version 27.9 Adobe Systems Software Ireland Limited https://www.adobe.com/products/catalog.html

Other

Viba-Brush Rover Medical Devices BV, Oss, The Netherlands https://www.roversmedicaldevices.com/cell-sampling-devices/viba-brush/
FTA Elute Micro Card GE Healthcare, Cardiff, UK art. no WB129308
Harris Micropunch 3 mm Whatman art.no WB100038
Protein LoBind tubes 1.5 mL Eppendorf https://www.sigmaaldrich.com/SE/en/product/sigma/ep0030108116
Speedvac system ISS110 ThermoFisher Scientific, Massachusetts, USA ThermoFisher Scientific
Q-Exactive Plus mass spectrometer Thermo Finnigan, Bremen, Germany https://www.thermofisher.com/order/catalog/product/IQLAAEGAAPFALGMBDK