Biological samples |
|
Cervico-vaginal fluid |
Homo sapiens |
N/A |
Plasma samples |
Homo sapiens |
N/A |
|
Chemicals, peptides, and recombinant proteins |
|
LC/MS grade water |
Fisher Chemical |
Cat#51140 |
DL-Dithiothreitol (DTT) |
Sigma-Aldrich |
Cat#43815 |
2-Iodoacetamide (IAA) |
Sigma-Aldrich |
Cat#I6125 |
Sequencing Grade Modified Trypsin |
Promega |
Cat#V5111 |
Acetonitrile, LC-MS grade, 99.8% |
Fisher Chemical |
Cat#047138.M1 |
Formic acid |
Sigma-Aldrich |
Cat#F0507 |
Ammonium bicarbonate, 99% |
Fisher Chemical |
Cat#393210010 |
|
Critical commercial assays |
|
Proximity Extention Assay, custom version. |
Olink Proteomics AB |
www.olink.com |
|
Deposited data |
|
Deposited data |
This paper |
https://doi.org/10.17044/scilifelab.24241237 |
Data published in another paper |
Gutiérrez, A. L. et al.20 Identification of Candidate Protein Biomarkers for CIN2+ Lesions from Self-Sampled, Dried Cervico–Vaginal Fluid Using LC-MS/MS. Cancers 2021, Vol. 13, Page 259213, 2592 (2021). |
https://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD026064 |
|
Software and algorithms |
|
MaxQuant software, version 1.5.1.2 |
https://www.biochem.mpg.de/6304115/maxquant |
https://www.maxquant.org/ |
R version 4.2.2 |
R Core Team (2022) |
https://www.R-project.org/ |
Adobe Illustrator version 27.9 |
Adobe Systems Software Ireland Limited |
https://www.adobe.com/products/catalog.html |
|
Other |
|
Viba-Brush |
Rover Medical Devices BV, Oss, The Netherlands |
https://www.roversmedicaldevices.com/cell-sampling-devices/viba-brush/ |
FTA Elute Micro Card |
GE Healthcare, Cardiff, UK |
art. no WB129308 |
Harris Micropunch 3 mm |
Whatman |
art.no WB100038 |
Protein LoBind tubes 1.5 mL |
Eppendorf |
https://www.sigmaaldrich.com/SE/en/product/sigma/ep0030108116 |
Speedvac system ISS110 |
ThermoFisher Scientific, Massachusetts, USA |
ThermoFisher Scientific |
Q-Exactive Plus mass spectrometer |
Thermo Finnigan, Bremen, Germany |
https://www.thermofisher.com/order/catalog/product/IQLAAEGAAPFALGMBDK |