FIG. 1.

Time course of hepatocyte CD14 mRNA induction during endotoxemia. Sprague-Dawley rats were injected with LPS (10 mg/kg, i.p.). Hepatocytes were isolated at 1.5, 3, 6, 12, and 24 h from either LPS-treated or control rats. Total RNA was extracted, and Northern blot analysis was performed for CD14 mRNA. Membranes were rehybridized with probe for 18S rRNA. The levels of CD14 mRNA were normalized to 18S rRNA and are presented as the fold increase over the controls. Each column represents the mean ± the SE of triplicate samples at each time point (∗, P < 0.05 versus control).