FIG. 2.

Steady-state CD14 mRNA levels in liver tissue after LPS injection. Sprague-Dawley rats were injected with LPS (10 mg/kg, i.p.). Total RNA was extracted from the liver at 1.5, 3, 6, 12, and 24 h after LPS injection, and Northern blot analysis was performed for CD14 mRNA. Membranes were rehybridized with a probe for 18S rRNA. The bar graph shows the levels of CD14 mRNA normalized to 18S rRNA and presented as the fold increase over the controls. The Northern blot is from a representative experiment. Each column represents the mean ± the SE of three independent experiments (∗, P < 0.05 versus control).