FIG. 5.

(A) CD14 protein levels in freshly isolated and cultured hepatocytes. Western blot procedures and abbreviations are as described for Fig. 4. Each group contains duplicate samples from individual animals. Lanes 1 and 2, freshly isolated control hepatocytes; lanes 3 and 4, freshly isolated hepatocytes from LPS-treated rats (10 mg/kg, i.p., 12-h treatment); lanes 5 and 6, control hepatocytes plated for 24 h; lanes 7 and 8, cultured hepatocytes from LPS-treated rats (10 mg/kg, i.p., 12-h treatment) plated for 24 h. (B) CD14 protein levels in hepatocyte culture supernatant. Each group contains duplicate samples from individual animals. Lanes 1 and 2, control hepatocytes plated for 24 h; lanes 3 and 4, cultured hepatocytes from LPS-treated rats (10 mg/kg, i.p., 12-h treatment) plated for 24 h. The data are representative of three independent experiments.