FIG. 8.

NF-κB activation by LPS in CHO/rCD14 cells in serum and serum-free conditions. The experiment was carried out as described in Materials and Methods. Cells were treated with different doses of LPS prior to nuclear protein extraction. The doses of LPS for treatment are indicated, except in CHO/neo cells (0.1 μg/ml). Abbreviations: CHO/neo, stably neotransfected CHO cells; p65 Ab, anti-NF-κB p65 subunit antibody; p50 Ab, anti-NF-κB p65 subunit antibody. The data are representative of two independent experiments.