Figure 4.

Behavioral phenotype characterization using PCA. PCA biplot of clustering nonanxious (green line) and anxious subjects (red line) from CAF (blue dots) and CON (purple dots) group in (A) F1, (D) F2, and (G) F3 generations. Phenotype pie charts describing the percentage of nonanxious and anxious subjects of each generation and diet group F1 (B,C), F2 (E,F), and F3 (H,I). The representative image depicting the inheritance of the phenotype based on PCA results contribution can be found in Extended Data Figure 4-1, while Extended Data Figure 4-2 contains the PCA contributions of variables.