Table 5.

Results of two-way ANOVA for prenatal diet and behavioral phenotype group comparison in brain region of F1 corresponding to Figure 5

Region	Model		Degree of freedom	Sum of squares	Mean square	F value	Adjusted p-value
CA3 Contrast CON-NA Vs CAF-A	CA3 ∼ PD * phenotype	PD	1	0.0329	0.0329	105.960	0.0001***
		Phenotype	1	0.0114	0.0114	36.791	0.0001***
		PD * phenotype	1	0.0002	0.0002	0.945	0.3539
Lb3 Contrast CON-A Vs CAF-A	Lb3 ∼ PD * phenotype	PD	1	0.1964	0.1964	429.879	0.0001***
		Phenotype	1	0.0339	0.0339	74.367	0.0001***
		PD * phenotype	1	0.0038	0.0038	8.459	0.0156*
Lb3 Contrast CON-NA Vs CAF-A	Lb3 ∼ PD * phenotype	PD	1	0.1964	0.1964	429.879	0.0001***
		Phenotype	1	0.0339	0.0339	74.367	0.0001***
		PD * phenotype	1	0.0038	0.0038	8.459	0.0156*
Lb3 Contrast CON-NA Vs CAF-NA	Lb3 ∼ PD * phenotype	PD	1	0.1964	0.1964	429.879	0.0001***
		Phenotype	1	0.0339	0.0339	74.367	0.0001***
		PD * phenotype	1	0.0038	0.0038	8.459	0.0156*
TH Contrast CON-NA Vs CAF-A	TH ∼ PD * phenotype	PD	1	0.0094	0.0094	10.069	0.0099**
		Phenotype	1	0.0258	0.0258	27.432	0.0003***
		PD * phenotype	1	0.0080	0.0080	8.565	0.01513*
IL Contrast CON-NA Vs CAF-NA	IL ∼ PD * phenotype	PD	1	0.1412	0.1412	257.68	0.0001***
		Phenotype	1	0.0450	0.0450	82.16	0.0001***
		PD * phenotype	1	0.0056	0.0056	10.26	0.0094*
HT Contrast CON-NA Vs CAF-NA	HT ∼ PD * phenotype	PD	1	0.0242	0.0242	58.855	0.0001***
		Phenotype	1	0.0005	0.0005	1.358	0.2710
		PD * phenotype	1	0.0015	0.0015	3.684	0.0839
PIR Contrast CON-NA Vs CON-A	PIR ∼ PD * phenotype	PD	1	0.0001	0.0001	0.072	0.7935
		Phenotype	1	0.0378	0.0378	19.724	0.0012**
		PD * phenotype	1	0.1422	0.1422	74.166	0.0001***

Behavioral traits in the offspring of mice prenatally exposed to HED. PD = distance traveled during open field.

^*<0.05.

^**<0.01.

^***<0.001.