Figure 3.
PEG-HCCs catalytically reduce both resazurin and ferricytochrome c (CytCox) with NADH to resorufin and ferrocytochrome c (CytCred), respectively. A) The reduction rate of resazurin in the presence of NADH is linearly related to the concentration of PEG-HCCs. B) The reduction rate of resazurin while holding the concentration of resazurin and PEG-HCCs constant varies linearly with the concentration of NADH. C) The reaction rates of NADH with resazurin and PEG-HCCs is not linearly related to the concentration of resazurin and no saturation point is observed within the detection limits of our instrumentation. D) Lineweaver-Burk plot of C showing intersecting lines suggesting the role of a ternary complex in the reduction of resazurin. E) NADH-linked reduction of CytCox to CytCred showing a saturation point at all three concentrations of NADH and full sigmoidal curve.