Skip to main content
. 2023 Dec 29;13(1):e031219. doi: 10.1161/JAHA.123.031219

Figure 1. Deferasirox (DFX) reduces hypoxia (Hx)‐induced iron accumulation in the endoplasmic reticulum (ER) in cultured cardiomyocytes.

Figure 1

A, Experimental protocol of hypoxia‐reoxygenation (H/R) for inducing ferroptosis in cultured cardiomyocytes. B, Representative images of cardiomyocytes treated with FerroFarRed (FFR; red) to detect iron in the ER. The ER and nuclei were co‐stained with ERseeing (green) and DAPI (blue), respectively. Scale bar: 10 μm. C, Quantification of the fluorescence intensity of FFR in FFR‐stained cardiomyocytes (n=20, each group). Data are presented as the mean±SEM. Statistical significance was determined using 1‐way ANOVA with Tukey post hoc test. ***P<0.001. 4‐HNE indicates 4‐hydroxynonenal; BODIPY, boron‐dipyrromethene; COX2, cyclooxygenase 2; Nx, normoxia; and Veh, vehicle.