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. 2023 Dec 29;13(1):e031219. doi: 10.1161/JAHA.123.031219

Figure 2. Deferasirox (DFX) suppresses lipid peroxidation caused by hypoxia/reoxygenation (H/R) in cultured cardiomyocytes.

Figure 2

A, Western blotting for proteins modified by 4‐hydroxynonenal (4‐HNE) and cyclooxygenase 2 (COX2). GAPDH represents the internal control. B, Quantification of 4‐HNE protein modifications (left panel) and COX2 expression (right panel; n=6, each group). C, Gene expression of Ptgs2, measured by reverse transcription‐quantitative polymerase chain reaction (n=6, each group). D, Representative flow cytometric analysis of cultured cardiomyocytes probed with C11 boron‐dipyrromethene (BODIPY) after H/R. E, Quantification of C11‐BODIPY–positive cells (n=6, each group). Data are presented as the mean±SEM. Statistical significance was determined using 1‐way ANOVA with Tukey post hoc test. *P<0.05, **P<0.01, ***P<0.001.