| Cell line (human) |
HEK293T |
ATCC |
ATTC:CRL-3216 |
|
| Cell line (human) |
Jurkat, Clone E6-1 |
ATCC |
ATTC:TIB-152 |
|
| Recombinant DNA reagent |
pNL4.3-iGFP-ΔEnv |
DOI: 10.1371/journal.ppat.1002762
|
|
|
| Recombinant DNA reagent |
psPAX2 |
NIH AIDS Reagent Program |
NIH AIDS Reagent Program:#11348 |
|
| Recombinant DNA reagent |
pCRV1-GagPol |
DOI: 10.1128/jvi.78.21.12058–12061.2004
|
|
|
| Recombinant DNA reagent |
pCSGW |
DOI: 10.1126/science.272.5259.263
|
|
Transfer vector encoding GFP |
| Recombinant DNA reagent |
pMD2.G |
Addgene (Trono lab) |
Addgene:#12259 |
Encodes VSV-G envelope protein |
| Recombinant DNA reagent |
pMCSG7-DLY |
DOI: 10.1128/iai.00927–12
|
|
Plasmid for bacterial expression of recombinant desulfolysin from Desulfobulbus propionicus |
| Sequence-based reagent |
2-LTR junction fwd |
Integrated DNA Technologies |
|
PCR primer for quantification of 2-LTR circles, 5’-GCTAACTAGGGAACCCACTGCTTAAG-3’ |
| Sequence-based reagent |
2-LTR junction rev |
Integrated DNA Technologies |
|
PCR primer for quantification of 2-LTR circles, 5’-ACTGGTACTAGCTTGTAGCACCATCCA-3’ |
| Sequence-based reagent |
Mf374 probe |
Sigma-Aldrich |
|
6-FAM-ACA [C]A[C]A[A]G[G][C]T-BHQ-1 |
| Sequence-based reagent |
KBrun692F |
Sigma-Aldrich |
|
PCR primer for quantification of viral DNA, 5’-CAGGACTCGGCTTGCTGAAG-3' |
| Sequence-based reagent |
KBrun797R |
Sigma-Aldrich |
|
PCR primer for quantification of viral DNA, 5’-GCACCCATCTCTCTCCTTCTAGC-3’ |
| Peptide, recombinant protein |
CPSF6p |
GenScript |
|
custom synthesised peptide, sequence: PVLFPGQPFGQPPLG |
| Peptide,- recombinant protein |
CPSF6p-Cys |
GenScript |
|
custom synthesised peptide, sequence: PVLFPGQPFGQPPLGC |
| Chemical compound, drug |
GS-6207 |
MedChemExpress |
MedChemExpress:HY-111964 |
|
| Chemical compound, drug |
PF74 |
Sigma-Aldrich |
Sigma-Aldrich:SML0835 |
|
| Chemical compound, drug |
BI-2 |
Enamine |
Enamine:Z1982491200 |
|
| Chemical compound, drug |
EZ-Link Sulfo-NHS-LC-LC-Biotin |
Thermo Fisher Scientific |
Thermo Fisher Scientific:21338 |
|
| Software, algorithm |
JIM Immobilized Microscopy analysis package |
Walsh, 2021
|
|
https://github.com/lilbutsa/JIM-Immobilized-Microscopy-Suite
|
| Other |
SLO |
Sigma-Aldrich |
Sigma-Aldrich:S5265-25KU |
Streptolysin O from Streptococcus pyogenes
|
