Figure 1.

The single-cell landscape of neuroblastoma

(A) Overview of patients and samples.

(B) UMAP of main cell types.

(C) Heatmap of top 5 differentially expressed genes per main cell type.

(D) UMAP showing expression of neuroblastoma-associated genes.

(E) Proportion of different cell types per sample. ^∗Only cells which were sorted with an unbiased FACS sorting strategy were included, which led to exclusion of M761AAA_T, M241AAE_T, and M259AAA_T. “T1” and “T2” refer to paired samples before and after treatment, respectively.

(F) Average cellular composition of samples before and after induction chemotherapy. Mixed-effects analysis with Sidak’s multiple comparisons test. ^∗p < 0.005.

(G) Proportion of mesenchymal and tumor cells in five paired pre- and post-treatment samples. Mann-Whitney U-test. Also see Figure S1.