Extended Data Fig. 6. Signal crosstalk validation of 5 thermal probes for 488 nm channel.

a, RNA FISH images for ARID1A targeting with Atto488-labeled imagers for the 39 °C thermal channel and RNA copies per cell for all fluorescent and thermal channels. There is no notable signal crosstalk between the different thermal channels. b, Validation for the thermal probe set targeting the DGKD transcript with Atto488-labeled imagers for the 48 °C thermal channel. c, Validation for FOSL2 transcript thermal probe set with Atto488-labeled imagers for the 57 °C thermal channel. d, Validation for NCOR1 transcript thermal probe set with Atto488-labeled imagers for the 65 °C thermal channel. e, Validation for APC transcript thermal probe set with Atto488-labeled imagers for the 72 °C thermal channel.