Extended Data Fig. 7. Signal crosstalk validation of 5 thermal probes for 565 nm channel.

a, RNA FISH images for BAZ1B target with Atto565-labeled imagers for the 39 °C thermal channel and RNA copies per cell for all fluorescent and thermal channels. There is no notable signal crosstalk between the different thermal channels. b, Validation for the thermal probe set targeting the EGFP transcript with Atto488-labeled imagers for the 48 °C thermal channel. c, Validation for HEG1 transcript thermal probe set with Atto488-labeled imagers for the 57 °C thermal channel. d, Validation for POGK transcript thermal probe set with Atto488-labeled imagers for the 65 °C thermal channel. e, Validation for NUP205 transcript thermal probe set with Atto488-labeled imagers for the 72 °C thermal channel.