Extended Data Fig. 8. Signal crosstalk validation of 5 thermal probes for 647 nm channel.

a, RNA FISH images for ARHGEF28 target with Alexa647-labeled imagers for the 39 °C thermal channel and RNA copies per cell for all fluorescent and thermal channels. There is no notable signal crosstalk between the different thermal channels. b, Validation for the thermal probe set targeting the ELAVL1 transcript with Alexa647-labeled imagers for the 48 °C thermal channel. c, Validation for FNDC3B transcript thermal probe set with Alexa647-labeled imagers for the 57 °C thermal channel. d, Validation for KIF1C transcript thermal probe set with Alexa647-labeled imagers for the 65 °C thermal channel. e, Validation for ITPR3 transcript thermal probe set with Alexa647-labeled imagers for the 72 °C thermal channel.