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β-Galactosidase activities in P. gingivalis tpr::lacZ chromosomal mutants. P. gingivalis W83, a single-crossover (SC) transconjugant, and a double-crossover (DC) transconjugant (P. gingivalis W83/lacZ) were grown in BHI or 0.5TYE. One unit of β-galactosidase activity is equivalent to hydrolysis of 1 nmol of ONPG min−1 mg of total cellular protein−1.
