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. 2024 Feb 13;15:1337. doi: 10.1038/s41467-024-45573-9

Fig. 1. Tyk2 deficiency suppresses autoimmune diabetes in NOD mice.

Fig. 1

a Incidence of spontaneous diabetes in female Tyk2+/+ (n = 14), Tyk2+/− (n = 10), and Tyk2−/− NOD mice (n = 13). Diabetes was defined by a non-fasting blood glucose level exceeding 250 mg/dL. b Insulitis scores of normoglycemic mice at 14w (Tyk2+/+, n = 8; Tyk2+/−, n = 8; Tyk2−/−, n = 8) and 24w (Tyk2+/+, n = 10; Tyk2+/−, n = 8; Tyk2−/−, n = 10). Score 0, no insulitis; Score 1, peri-insulitis; Score 2, infiltrative insulitis less than 50% of the islet area; and Score 3, infiltrative insulitis more than 50% of the islet area (Supplementary Fig. 1d). c Insulin autoantibody levels in the serum of normoglycemic mice at 14w (Tyk2+/+, n = 17; Tyk2+/−, n = 22; Tyk2−/−, n = 21) and 24w (Tyk2+/+, n = 10; Tyk2+/−, n = 8; Tyk2−/−, n = 16). d Incidence of diabetes in female wild type NOD.SCID mice adoptively transferred with splenocytes from normoglycemic female 14w Tyk2+/− NOD mice (n = 7), 14w Tyk2−/− NOD mice (n = 7), or 24w Tyk2−/− NOD mice (n = 10). Splenocytes (1 ×107) were intravenously transferred into 6–8w recipient mice. Diabetes was defined by a non-fasting blood glucose level exceeding 250 mg/dL. e Incidence of diabetes in female Tyk2−/− NOD.SCID mice adoptively transferred with splenocytes (1 ×107) from normoglycemic female 14w Tyk2+/− NOD mice (n = 8), 14w Tyk2−/− NOD mice (n = 6), or 24w Tyk2−/− NOD mice (n = 8). Data represent the mean ± SEM. P-values were calculated using the log-rank test (a, d, e), and one-way ANOVA with Tukey’s posttest (b, c).