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. 2024 Feb 13;15:1337. doi: 10.1038/s41467-024-45573-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a (Left) Representative flow cytometry plots and (right) graph indicate the frequency of CD44^hi T-BET⁺ polyclonal cells among CD8⁺ T cells in the PLN of 6w female mice (Tyk2^+/+, n = 6; Tyk2^+/−, n = 12; Tyk2^−/−, n = 7). b Frequency of CD44^hi T-BET⁺ cells among IGRP-specific CD8⁺ T cells in the PLN of 6w female mice (Tyk2^+/−, n = 8; Tyk2^−/−, n = 6). c Protein expression levels of T-BET in CD44^hi T-BET⁺ IGRP-specific CD8⁺ T cells in the PLN of 6w female mice (Tyk2^+/−, n = 9; Tyk2^−/−, n = 6). Frequency of CD44^hi T-BET⁺ cells (d) and protein expression levels of T-BET (e) in unstimulated or ex vivo-activated Tyk2^+/− or Tyk2^−/− CD8⁺ T cells with anti-CD3/CD28 beads and IL-2 in the presence or absence of IL-12 for 3d (Tyk2^+/−, n = 4; Tyk2^−/−, n = 4). f (Left) Representative flow cytometry plots and (right) graph indicate the frequency of CD44^hi CXCR3⁺ cells among polyclonal CD8⁺ T cells in the PLN of 6w female mice (Tyk2^+/+, n = 7; Tyk2^+/−, n = 5; Tyk2^−/−, n = 7). g Frequency of CD44^hi CXCR3⁺ cells among IGRP-specific CD8⁺ T cells in the PLN of 6w female mice (Tyk2^+/−, n = 7; Tyk2^−/−, n = 5). h PCA of transcriptome data of purified CD44^hi IGRP-specific CD8⁺ T cells in the PLN (Tyk2^+/+, n = 3; Tyk2^−/−, n = 3) and IGRP-specific CD8⁺ T cells in the pancreas (Tyk2^+/+, n = 3; Tyk2^−/−, n = 3). Gene expression profiles in the purified cells were analyzed by RNA-seq analysis. i Box plots show the pattern of differentially expressed genes in IGRP-specific CD8⁺ T cells in the PLN (Tyk2^+/+, n = 3; Tyk2^−/−, n = 3) and pancreas (Tyk2^+/+, n = 3; Tyk2^−/−, n = 3). Likelihood ratio tests were used to determine differentially expressed genes between the genotypes (FDR < 0.1, fold change >1.5). Selected genes are highlighted on the right side of each box plots. Boxes and whiskers represent the median and the lower and upper hinges correspond to the 25th and 75th percentiles. The whiskers extend from the hinges to the values no further than 1.5 times the inter-quartile ranges from each box hinge. Data represent the mean ± SEM. P-values were calculated using one-way ANOVA with Dunnett’s posttest (a, f) and two-tailed Student’s t test (b–e, and g).