FIG. 5.

SHelAg-induced cytokine responses of spleen cells from H. hepaticus-infected WT and IL-10^−/− mice. Spleen cells (5 × 10⁶/ml) from uninfected (Uninf.) and 9-week-infected (Inf.) WT and IL-10^−/− mice were stimulated with medium alone (□) or 1 μg of SHelAg per ml (■), and IL-10 (A), IFN-γ (B), TNF-α (C), and NO (D) were measured in 72-h supernatants. Bars represent means ± SD of values obtained from two separate pools of mice per group tested in duplicate (three females and three males in each pool). Data for one of seven experiments (using 4-, 6-, 9-, 10-, or 11-week-infected mice) with similar results are shown.