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. 2024 Feb 13;15:1341. doi: 10.1038/s41467-024-45532-4

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© The Author(s) 2024, corrected publication 2024

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Fig. 4 — A Graphical representation of the IMD pathway. B RNAi-mediated PGRP-LC (UAS-pgrp-LC RNAi) inactivation in the Gr66a+ or ppk23+ cells does not affect PGN-triggered aversion. C RNAi-mediated Myd88 (UAS-Myd88 RNAi) inactivation in the Gr66a+ or ppk23+ cells does not affect PGN-triggered aversion. D RNAi-mediated TRPA1 (UAS-TRPA1 RNAi) inactivation in the Gr66a+ cells abrogates PGN-induced aversion, while its inactivation in ppk23+ cells has no effect on the aversion phenotype. E The nociceptive TRPA1 channel is required in the larval stages for PGN-induced aversion, while its RNAi dependent inactivation in the adult stage has no effect on PGN-triggered aversion. The ubiquitously expressed Tub-Gal80^ts, that inhibits the activity of Gal4, is temperature sensitive: it’s active at 18 °C and inactivated at 29 °C, allowing the expression of TRPA1-RNAi and the consequent inactivation of TRPA1 in Gr66a+ cells. For (B–E) PER index of flies to control solutions of sucrose 1 mM and sucrose 1 mM + caffeine 10 mM and to sucrose 1 mM + PGN from E. coli K12 at 200 µg/mL. PER index is calculated as the percentage of flies tested that responded with a PER to the stimulation ± 95% CI. The number of tested flies (n) is indicated on top of each bar. ns indicates p > 0.05, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001, **** indicates p < 0.0001 two-sided Fisher Exact Test. Further details including raw data and exact p-values can be found in the source data file.