Fig. 3.

Zbtb16 or Txnip knockdown reduced combination of Txnip-Trx2 and improved Trx2 function. A Immunofluorescence co-staining for α-Actinin with Flag in mouse heart. B, C The respective WB images and quantitative analysis of Zbtb16 expression (B) in db/ + and db/db mouse atrial myocytes and Txnip expression (C) in mitochondria (n = 6 for each group). D, E co-IP assay of Txnip-Trx2 combination in mitochondria after Zbtb16-KD (D) or Txnip-KD (E) (n = 6 for each group). F Analysis of Trx2 activity in mitochondria of db/ + and db/db mouse atria (n = 6 for each group). Bar = 1 μm. Data in (B, C, and F) were analyzed by two-way ANOVA with Bonferroni’s multiple comparisons test. KD knockdown