Fig. 6.

Zbtb16 or Txnip knockdown ameliorated Trx2 activity and reduced ROS-induced CaMKIIδ activation in HL-1 cells. A, B co-IP assay of Txnip-Trx2 combination in mitochondria after Zbtb16-KD (A) or Txnip-KD (B) in HL-1 cells (n = 6 for each group). C Analysis of Trx2 activity in mitochondria of HL-1 cells (n = 6 for each group). D, E Representative images (D) and quantitative analysis (E) of ROS level measured by DHE staining in HL-1 cells (n = 18). Bar = 200 μm. F–J Representative WB images (F) and quantitative analysis in HL-1 cells of CaMKIIδ (G), RyR2 (H), PLB (I), and SERCA2a (J) with Zbtb16- KD, Txnip-KD or Mito-TEMPO treatment (n = 6 for each group). Data in (C, E and G–J) were analyzed by two-way ANOVA with Bonferroni’s multiple comparisons test. KD knockdown