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Inhibition of IgG binding to solid-phase coated nAsp f 2. Polyclonal mouse sera raised against nAsp f 2 AF102 (▵), nAsp f 2 AF104 (□), and rAsp f 2 (○) were preincubated overnight with different amounts of rAsp f 2 as indicated on the x axis. Preincubated serum samples were transferred to nAsp f 2 (AF104)-coated wells, and antibody binding was measured.
